see method in this website:
http://flysnp.imp.ac.at/methods.html#isolation
Here is a copy of the pdf file content:
Single fly genomic DNA preparation This is a short protocol for the isolation of genomic DNA from a single fly for PCR applications. Of the DNA yield, 1-5µl were used to perform PCR reactions. Squishing Buffer (SB): 10mM Tris-HCl pH8.2 1mM EDTA 25mM NaCl 200µg/ml Proteinase K (store 4mg/ml stock solution in –80˚C and add 1/20 to the SB just before use) 1. Add Proteinase K to the Squishing Buffer (SB) freshly from the stock. 2. Place each single fly in wells of a 96 well plate and mash the fly for 5-10 seconds with the tips of a multi-pipette containing 50µl of SB each, without expelling any liquid (sufficient liquid escapes from the tip). Expel the remaining SB. 3. Incubate at 37˚C for 30 minutes. 4. Inactivate the Proteinase K by heating to 95˚C for 5 minutes. It is convenient to use the PCR machine. 5. Add 50µl of TE or H2O. Use 1µl for PLP-PCR (~200bp), or 2-5µl for normal PCR (~1kb).
